Induction of Indoleacetic Acid Synthetases in Tobacco Pith Explants

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Comparative studies on tobacco pith and sweet potato root isoperoxidases in relation to injury, indoleacetic Acid, and ethylene effects.

Sweet potato (Ipomoea batatas) root parenchyma and tobacco (Nicotiana tabacum) stem pith, both known to increase peroxidase activity after excision, differed from each other in their isoperoxidase patterns and in the isoperoxidase responses to injury and exogenous ethylene.In potato root sections, the injury-dependent peroxidase increase was due to an induction of two isoenzymes, as well as to ...

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Promotion of indoleacetic Acid oxidase isoenzymes in tobacco callus cultures by indoleacetic Acid.

Indoleacetic acid oxidase in tobacco callus cultures (Nicotiana tabacum L., cv. White Gold) was composed of at least two groups of isoenzymes, which were distinctly different in electrophoretic mobilities and in responses to growth substances. Indoleacetic acid had dual effects; at low concentrations it promoted the development of two fast-migrating indoleacetic acid oxidase isoenzymes, but at ...

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Changes in indoleacetic Acid oxidase isoenzymes in tobacco tissues after treatment with 2,4-dichlorophenoxyacetic Acid.

2,4-Dichlorophenoxyacetic acid had a multiple effect on the development of indoleacetic acid oxidase isoenzymes in tobacco callus tissues (Nicotiana tabacum, cv. White Gold) cultured in vitro, and the development of these isoenzymes was differentially associated with growth promotion or inhibition depending on the concentration of 2,4-dichlorophenoxyacetic acid. At low concentrations (0.1 to 1 ...

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Colorimetric Estimation of Indoleacetic Acid.

The wide use of the auxin, indoleacetic acid, in physiological and biochemical experiments has promoted interest in methods for its colorimetric estimation. MITCHELL and BRUNSTETTER (1) have proposed both the nitrite and the ferric chloride-sulphuric acid tests for the quantitative estimation of indoleacetic acid (IAA) in aqueous solutions, basing their suggested procedures upon a study of opti...

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Structural physiological, and biochemical gradients in tobacco pith tissue.

Explants of tobacco pith taken at various distances from the apex of a mature stem show a sharp gradient in growth potential in vitro; growth is highest in the extreme apical and basal explants, and is minimal in explants removed ca. 75 cm from the apex. Calluses produced by the vigorously growing basal explants are harder and more compact than those produced from more apical explants. The grad...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1972

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.50.6.723